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Searching PINT

Welcome to the PINT database. This page contains information on how to search the PINT database. If you are a new user please go through these pages before attempting to search the PINT database.

There are two kinds of search available in PINT. The Quick search and the Advanced search.

Enter a keyword and hit the search button. "Quick search" searches only the text fields. Please note that you have to enter a full word to get a result. To find a protein "Lysozyme", you must enter the full word "Lysozyme". You won't get any result if you enter "Lyso" or "Lysozy" etc. Wildcard characters will NOT work in "Quick Search".

Advanced search:

Advanced search page has different fields to choose from. For most of the fields we have provided a drop down menu to help the users. We believe that this will save time and will avoid unwanted entries in the fields.
There are three steps to get the data.

1. Choose the necessary search options from the drop down menu, or enter the search options in the fields.
2. Select the display options. These selected fields will be displayed in the search result page. Please note that we have selected some fields as a default. Use the reset button to get back our default settings.

Explanation of the terms in the Advanced Search and query tips:


PINT Code:

For each protein-protein interaction between two particular proteins, we have assigned a code, PINT code. This is mainly to identify the interaction between two particular proteins. The template for this code is PINT_XXXX_YYYY. Where PINT: represent our database, XXXX and YYYY are the interacting proteins.
For example: The PINT code for the interaction between Barnase and Barstar is given as: PINT_BARN_BARS. This code, PINT_BARN_BARS is assigned to all the interaction data between these two proteins. This search option is provided as a drop down list. User may select the appropriate PINT code to get the interaction data between two proteins. Please see the PINT Code List to see all the codes in the database.

The name of the interacting protein. Select the protein name that has to be queried from the drop down list. This list contains all the proteins presently available in this database.
Please not that, we have assigned the protein and peptide names based on the experiments, not based on the protein length.
For example: For ITC experiments, we have assigned the reactant inside the reaction cell as the protein and the reactant getting injected as peptide.
So if you don't find the particular protein you are looking for under the "Protein name" field, please check under the "Peptide name" field also. Please see the Protein Name List available in PINT database.

Source (Organism) of the interacting protein. Select the protein source that has to be queried from the drop down list. Please see the Protein Source List available in PINT database.

The domain or the fragment of the interacting proteins.

The PIR-PSD code of the interacting protein. Select the PIR code that has to be queried from the drop down list. Please see the PIR List available in PINT database. Please see the PIR Code List available in PINT database.

The Swiss-Prot ID of the interacting protein. Select the Swiss-Prot ID that has to be queried from the drop down list. Please see the Swiss-Prot List available in PINT database. Please see the Swiss-Prot List available in PINT database.

The PDB code of the interacting protein. Select the PDB code that has to be queried from the drop down list. Please see the PDB List available in PINT database.

Mutation of the protein/peptide. Single: Single mutation, Double: double mutation, Multiple: Multiple mutation, Wild: no mutation (wild protein). Multiple boxes can be checked to search for single, double, multiple mutations and wild proteins.

Secondary structure information of the residue at the mutant position. We have used the DSSP program to assign the secondary structure information. Multiple boxes can be checked to search for Helix (H), Strand (E), Bend (S), Turn (T) and Coil (C).

Accessible surface area (ASA) of the residue in wild type, obtained using the DSSP program, expressed in A2.

The name of the interacting peptide. Select the peptide name that has to be queried from the drop down list.
Please not that, we have assigned the protein and peptide names based on the experiments, not based on the protein length.
For example: For ITC experiments, we have assigned the reactant inside the reaction cell as the protein and the reactant getting injected as peptide.
So if you don't find the particular protein you are looking for under the "Peptide name" field, please check under the "Protein name" field also. Peptide Name List

Source (Organism) of the interacting protein. Select the protein source that has to be queried from the drop down list. Please see the Peptide Source List available in PINT database.

The domain or the fragment of the interacting proteins.

The Protein Data Bank (PDB) code of the interacting protein-protein complex. Select the PDB code that has to be queried from the drop down list. Please see the PDB-COMPLEX List available in PINT database.

The temperature (in degree celsius) at which the measurements were performed. Enter the desired T values in the text fields.
For example: To retrieve data for T values between 20 and 30 oC (including 20 and 30), enter both the values as To oC. To retrieve data for T values above 20 (including 20), enter the value only in the first text field and leave the second field blank, as shown. To oC. To retrieve data for T values below 35 (including 35), enter the value only in the second text field and leave the first field blank, as shown. To oC.

The pH value at which the measurements were made. Enter the desired pH values in the text fields.
The query has to be entered as explained in the case of Temperature.

The concentration of the protein used in the experiment. Enter the desired concentration values in the text fields.
The query has to be entered as explained in the case of Temperature.

The concentration of the peptide used in the experiment. Enter the desired concentration values in the text fields.
The query has to be entered as explained in the case of Temperature.

The buffer used in the experiment. Select the buffer that has to be queried from the drop down list. Please see the BUFFER List available in PINT database.

The ion used in the experiment. Select the ion that has to be queried from the drop down list. Please see the ION List available in PINT database.

The additives used in the experiment. Select the additives that has to be queried from the drop down list. Please see the ADDITIVES List available in PINT database.

Experimental method used for measuring the thermodynamic parameters. Select the method that has to be queried from the drop down list.

Dissociation constant for protein-protein interaction, in molar [M]. Enter the desired Kd values in the text fields.
For example: To retrieve data for Kd values between 2 x 10-9 and 10 x 10-9 (including both the values), enter the values as E To E M. The exponential part has to be selected from the drop down list. To retrieve data for Kd values above 2 x 10-9 (including this value), enter the value only in the first text field and leave the second field blank. To retrieve data for Kd values below 10 x 10-9 (including this value), enter the value only in the second text field and leave the first field blank.

The change in dissociation constant for protein-protein interaction for the wild and mutant proteins (Kdmutant - Kdwild), in molar [M]. Enter the desired dKd values in the text fields.
The query has to be entered as explained in the case of Kd.

Association constant for protein-protein interaction, in molar [M]. Enter the desired Ka values in the text fields.
The query has to be entered as explained in the case of Kd.

The change in association constant for protein-protein interaction for the wild and mutant proteins (Kamutant - Kawild), in molar [M]. Enter the desired dKd values in the text fields.
The query has to be entered as explained in the case of Kd.

Free energy change for the protein-protein interaction, in kcal/mol. Enter the desired dG values in the text fields.
The query has to be entered as explained in the case of Temperature.

The change in free energy for protein-protein interaction for the wild and mutant proteins (dGmutant - dGwild), in kcal/mol. Enter the desired ddG values in the text fields.
The query has to be entered as explained in the case of Temperature.

Enthalpy change for the protein-protein interaction, in kcal/mol. Enter the desired dH values in the text fields.
The query has to be entered as explained in the case of Temperature.

Heat capacity change for the protein-protein interaction, in kcal/mol/K. Enter the desired dCp values in the text fields.
The query has to be entered as explained in the case of Temperature.

Enter the keyword that has to be queried.

Select the author name from the drop down list that has to be queried. Please see the AUTHOR List available in PINT database.

Select the journal name from the drop down list that has to be queried. Please see the REFERENCE List available in PINT database.

Select the journal name from the drop down list that has to be queried.

Select the starting and ending year from the drop down menu.

Select the field that has to be sorted from the drop down list.

Select the order (ASCENDING or DESCENDING) by which the data has to be sorted.



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