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Protein–Protein Interactions Thermodynamic Database

Protein–protein interactions (PPIs) are fundamental to numerous biological processes, including signal transduction, gene expression regulation, immune response, and antibody-antigen binding. Understanding these interactions at the structural and thermodynamic level is essential for deciphering binding specificity, functional mechanisms, and molecular behavior.

To address this need, we have developed PINT, a curated Protein–Protein Interactions Thermodynamic Database that integrates experimental data, structural information, sequence details, and literature references for thousands of protein complexes.

What PINT Provides

PINT contains comprehensive experimental data on thermodynamic parameters for protein–protein interactions, combined with detailed sequence and structural information and experimental conditions. Each entry in the database includes:

  • Protein details: Names and source organisms of interacting proteins
  • Sequence codes: PIR, SWISS-PROT, and PDB accession numbers
  • Structural properties: Secondary structure and solvent accessibility of mutant positions
  • Experimental conditions: Buffers, pH, temperature, ions, and additives
  • Thermodynamic parameters: Binding free energy (ΔG), enthalpy (ΔH), entropy (ΔS), heat capacity change (ΔCp), and association/dissociation constants (Ka/Kd)
  • Literature references: Experimental data linked to peer-reviewed studies

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Search and Explore

PINT is designed for ease of use and efficiency:

  • Text Search: Quickly find specific proteins or interactions by name or accession code.
  • Advanced Search: Filter by experimental conditions, thermodynamic parameters, protein source, or structural features to extract precise datasets.

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Tutorials and Support

A dedicated tutorial section guides users through the database interface, demonstrating how to:

  • Optimize queries for accurate results
  • Retrieve detailed protein–protein interaction data
  • Apply thermodynamic and structural information in research workflows